Ionizing radiation-induced invasive phenotype: histamine actions in MCF-7 cells.

T. GALARZA, M. TÁQUEZ DELGADO, N MOHAMAD, V. MEDINA, G. MARTÍN, G CRICCO

Congreso; European Histamine Research Society. 44th Annual Meeting; 2015

A great deal of evidence shows the capability of ionizing radiation to enhance the migratory and invasive abilities of epithelial cancer cells through the acquisition of mesenchymal features promoting an invasive phenotype. We have previously demonstrated that histamine is able to block mesenchymal changes induced by 2 gray irradiation in pancreatic adenocarcinoma and triple negative breast cancer cells.The aim of this work was to investigate further histamine role in the ionizing radiation-induced invasive phenotype in the breast luminal cancer line MCF-7.Cells were cultured for 24, 48 or 110 h with or without different doses of histamine and irradiated or not with a dose of 2 gray of gamma radiation, which is usually used in fractionation radiotherapy.In non-irradiated cells, a concentration dependent effect was detected. Histamine concentrations between 0.5 and 5 µM produced cells with spindle shaped morphology (observed by optical microscope) and a decrease in the expression of the epithelial marker E-cadherin by immunoblot. Besides, a significant increase in the expression of mesenchymal markers: vimentin and slug by western blot (p