Histamine regulates the MAPK pathway via the H2 receptor in PANC-1 human cells

CRICCO G; MARTIN G; MEDINA V; GUTIÉRREZ A; NÚÑEZ M; COCCA C; BERGOC R; RIVERA E

INFLAMMATION RESEARCH

Birkhauser Verlag

Lugar: Basel; Año: 2004 vol. 53 p. 65 - 66

1023-3830

Histamine is a selective protector against cellular damaged produced by ionizing radiation VA Medina1 GP Cricco1 N Mohamad1 M Croci2 M Nuñez1 G Martin1 C Cocca1 RM Bergoc1 ES Rivera1Radioisotopes Laboratory, School of Pharmacy and Biochemistry, University of Buenos Aires Junin 956, 1113 Buenos Aires, ARGENTINA1Immunooncology Institute, 3200 Cordoba Av., 1187 Buenos Aires, ARGENTINA2 The present work investigated the capacity of HA to modulate the oxidative damaged produced by ionizing radiation. In order to study the effect of histamine (HA) on radiosensitivity of transformed cells, MDA-231 (human breast carcinoma cells) were irradiated in vitro with doses ranging from 0 to10 Gy employing a 137Cs source of 189 TBq (Dose rate: 7.7 Gy/min). HA from 0.1 to 10 mM was added to cultures 20 hs before irradiation. The survival curves were adjusted using the Lineal-Quadratic model. The levels of the free radicals superoxide (O2·-) and hydrogen peroxide (H2O2) were determined by flow citometry employing specific fluorescence staining. The activity of the antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) was also determined in MDA cells treated with HA 10 mM and/or irradiated with 2Gy.HA treatment produced a significant dose dependent decrease in survival of irradiated cells (p