Histamine production in mouse epidermal keratinocytes is regulated during cellular differentiation

C. FITZSIMONS, N ENGEL, H DURÁN, L POLICASTRO, G CRICCO, G MARTIN, B MOLINARI AND E RIVERA

INFLAMMATION RESEARCH

BIRKHAUSER VERLAG AG

Lugar: BASEL; Año: 2001 vol. 50

1023-3830

Cultured keratinocytes can be induced to undergo morphologicaland functional differentiation by modulating theextracellular [Ca2+]. This in vitro response correlates withthat observed in vivo as keratinocytes migrate from the basementmembrane toward the suprabasal differentiating layersof the epidermis . The elevation of extracelular Ca2+increases phosphatidylinositol turnover and diacylglycerollevels and induces protein kinase C activity, resulting in theexpression of differentiation markers such as transglutaminaseK (TGK) . We have previously demonstrated thatH2 receptor expression is regulated during differentiation ofa mouse keratinocyte-derived cell line (PB cells) and that thisaffects the functional coupling of the receptor to signal transductionpathways .In the present work, we studied changes in HDC expression,intracellular histamine content and histamine releaseinto the extracellular medium during Ca2+-induced epidermaldifferentiation of a mouse keratinocyte-derived cell line.