Oxidative stress response of scallop Aequipecten tehuelchus from Patagonia Argentina exposed to inorganic arsenic

STURLA LOMPRÉ, JULIETA; GIARRATANO, ERICA; GIL, MÓNICA NOEMÍ; BERTRAND, LIDWINA; MALANGA, GABRIELA

Regional Studies in Marine Science

Elsevier

Año: 2023 vol. 62

2352-4855

Laboratory studies were performed to assess the impact of acute arsenic (As) exposure on theTehuelche scallop Aequipecten tehuelchus from San José gulf (Patagonia, Argentina). The As accumulationin gills, digestive gland and muscle of A. tehuelchus was analyzed after 96 h of exposure at differentconcentrations (0, 4, 5, 6.3 and 7.9 mg As/L). Accumulation in all tissues increased linearly with theexposure concentration, evidencing no As saturation. A 96 h median lethal concentration (LC50) valueof 7.1 mg As/L was determined, characterizing this species as relatively tolerant to As. The potentialeffects of As were studied through the production of reactive species, enzymatic activities of catalaseand glutathione-S-transferase, metallothioneins, lipid peroxidation, and lipid-soluble antioxidants. Inpresence of As, the 2’,7’-dichlorodihydrofluorescein diacetate oxidation rate and thiobarbituric acidreactive substances content did not show changes in any tissues. Catalase and glutathione S-transferaseactivities raised in gills and digestive gland and remained unchanged in muscle. Metallothioneinsincreased in gills and digestive gland and a decreasing pattern of α-tocopherol was observed ingills and muscle. Scallops were slightly affected by As exposure, especially at high levels, being ableto counteract its effects by the induction of biotransformation pathways and antioxidant defensemechanisms