Screening of DERA activity in bacteria: Whole cell biocatalysed synthesis of 2-deoxyriboses-5-phosphate

ANA LAURA VALINO; MARTÍN PALAZZOLO; ELIZABETH LEWKOWICZ; ADOLFO IRIBARREN

Berna

Congreso; Biotrans2009; 2009

Sociedad Europea de Biocatálisis

The synthesis of 2-deoxyribose-5-phosphate (DR5P) is a key step in the preparation of deoxynucleosides1 that are usually chemically synthesised through several and complex steps. Therefore, the study of simpler, cleaner and more economic alternative paths becomes highly interesting. Aldolases catalyse the stereoselective C-C bond formation through a condensation reaction that involves carbonylic compounds. Among them, 2-deoxyribose-5-phosphate aldolase (DERA) is the only aldolase that accepts two aldehydic substrates2 to produce DR5P: D-glyceraldehyde-3-phosphate (G3P) and acetaldehyde. With the aim of selecting microorganisms containing DERA activity, our bacteria collection was screened. Ten microorganisms belonging to Bacillus, Xanthomona, Streptomyces and Erwinia genera metabolised 2-deoxyribose and tolerated high acetaldehyde concentration. These bacteria were tested for DR5P production using different starting material for G3P in situ generation. Consequently, unreported C74 strain from the Erwinia genus demonstrated to be an efficient biocatalyst for DR5P biosynthesis through a simple and clean process. Finally, the proposed biotransformation was assessed for the preparation of 2-b-substituted-2-deoxyriboses-5-phosphate from different aldehyde donors.