INVESTIGADORES
LEWKOWICZ Elizabeth Sandra
artículos
Título:
N-Acetylneuraminic acid aldolase-catalyzed synthesis of acyclic nucleoside analogues carrying a 4-hydroxy-2-oxoacid moiety
Autor/es:
NIGRO, MARIANO J.; MARTÍN PALAZZOLO; DIEGO COLASURDO; ADOLFO M. IRIBARREN; ELIZABETH LEWKOWICZ
Revista:
CATALYSIS COMMUNICATIONS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2019 vol. 121 p. 73 - 77
ISSN:
1566-7367
Resumen:
N-Acetylneuraminic acid aldolase (NeuAcA) (EC 4.1.3.3) is a pyruvate-dependent class I aldolase that catalyzes the reversible aldol cleavage of N-acetylneuraminic acid to form N-acetyl-D-mannosamine and pyruvate. The synthetic activity of this enzyme has been applied to the preparation of many sialic acid analogues. In this report, we demonstrate the ability of NeuAcA from Clostridium perfringens to use 2-oxoethyl substituted nucleobases as unusual acceptor substrates to perform aldol additions. In this way, novel acyclic nucleoside analogues bearing a further derivatizable 4-hydroxy-2-oxo butyrate skeleton were prepared in good yields.
