Selection of a new whole cell biocatalyst for the synthesis of 2-deoxyribose 5-phosphate

ANA LAURA VALINO; MARTÍN PALAZZOLO; ADOLFO IRIBARREN; ELIZABETH LEWKOWICZ

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

HUMANA PRESS INC

Año: 2012 vol. 166 p. 300 - 308

0273-2289

2-Deoxyribose 5-phosphate (DR5P) is a key intermediate in the biocatalyzed preparation of deoxyribonucleosides. Therefore, DR5P production by means of simpler, cleaner, and economic pathways becomes highly interesting. One strategy involves the use of bacterial whole cells containing DR5P aldolase as biocatalyst for the aldol addition between acetaldehyde andD-glyceraldehyde 3-phosphate or glycolytic intermediates that in situ generate the acceptor substrate. In this work, diverse microorganisms capable of synthesizing DR5P were selected by screening several bacteria genera. In particular,Erwinia carotovoraATCC 33260 was identified as a new biocatalyst that afforded 14.1-mM DR5P starting from a cheap raw material like glucose