Microbial synthesis of antiviral nucleosides using E. coli BL 21 as biocatalyst,

M.C. ROGERT; JORGE TRELLES; SILVIA PORRO; ELIZABETH LEWKOWICZ; ADOLFO IRIBARREN

BIOCATALYSIS AND BIOTRANSFORMATION

Taylor and Francis

Lugar: USA; Año: 2002 vol. 20 p. 347 - 351

1024-2422

Experimental conditions such as shaking (aeration) rate, concentration of reagents and extent of culture growth for the optimal synthesis of adenosine using Escherichia coli BL21 as biocatalyst were assessed, achieving 95% yield in 30 min of reaction using microorganisms harvested from late exponential phase. The ability of E. coli BL21 to synthesise purine nucleosides containing sugar residues such as 20-deoxyribose, 20,30-dideoxyribose and arabinose was also verified. 20-Deoxyribo- and arabinonucleosides could be prepared in high yield, while the results obtained with 20,30-dideoxyribonucleosides were not satisfactory. In the case of 20-deoxyadenosine, using thymidine as a starting material, a yield of 94% was achieved at 458C. Escherichia coli BL21 as biocatalyst were assessed, achieving 95% yield in 30 min of reaction using microorganisms harvested from late exponential phase. The ability of E. coli BL21 to synthesise purine nucleosides containing sugar residues such as 20-deoxyribose, 20,30-dideoxyribose and arabinose was also verified. 20-Deoxyribo- and arabinonucleosides could be prepared in high yield, while the results obtained with 20,30-dideoxyribonucleosides were not satisfactory. In the case of 20-deoxyadenosine, using thymidine as  starting material, a yield of 94% was achieved at 458C.