Aeromonas hydrophila strains as biocatalysts in the transglycosylation reaction

MATÍAS NÓBILE; MARCO TERRENI; ELIZABETH LEWKOWICZ; ADOLFO IRIBARREN

BIOCATALYSIS AND BIOTRANSFORMATION

TAYLOR & FRANCIS LTD

Año: 2010 vol. 28 p. 395 - 402

1024-2422

Microbial transglycosylation is useful as a green alternative in the preparation of purine nucleosides and analogues, especially for those that display pharmacological activities. In our constant search for new transglycosylation biocatalysts, two Aeromonas hydrophila strains were selected. The substrate specificity of both microorganisms was studied and as a result, several nucleoside analogues were prepared. Among them, ribavirin, a broad spectrum antiviral, and the well-known anti HIV didanosine, were prepared, in 77 and 62 % yield using A. hydrophila CECT 4226 and A. hydrophila CECT 4221 respectively. In order to scale-up the processes, the reaction conditions, the product purification as well as the biocatalyst preparation were analyzed and optimized.