Antioxidant response and oxidative stress levels in Macrobrachium borellii (Crustacea: Palaemonidae) exposed to the water-soluble fraction of petroleum

LAVARÍAS, S.; HERAS, H.; PEDRINI, N.; TOURNIER, H.; ANSALDO, M.

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. TOXICOLOGY & PHARMACOLOGY

ELSEVIER SCIENCE INC

Año: 2011 vol. 153 p. 415 - 421

1532-0456

The aim of the present work was to evaluate the effect of the water soluble fraction of hydrocarbons (WSF) onthe antioxidant status of the freshwater prawn Macrobrachium borellii. First, seasonal variations were studiedin a non-polluted area. Hepatopancreas and gills showed season-related fluctuations in catalase (CAT),glutathione-S-transferase (GST) activities and in lipid peroxidation levels (LPO), but not in superoxidedismutase (SOD). Then, adults were exposed semi-statically to sublethal doses for 7 days. CAT, SOD, GST, andglutathione peroxidase (GPx) activities and LPO, reduced glutathione (GSH) and protein oxidation (PO) levelswere determined. Exposed individuals showed significant increases in CAT, SOD, and GST activities inhepatopancreas and CAT activity in gills. GPx activity did not vary in either tissues. While LPO levels increased,GSH levels decreased significantly in hepatopancreas of exposed animals, but PO levels showed no variation.Induction of SOD was also assessed by Real-time PCR mRNA expression in hepatopancreas. The nonenzymaticantioxidant activity was also tested; ABTS 2,2′-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)was higher in hemolymph of treated-prawns compared to controls, but ferric reducing activity of plasmaassay (FRAP) values did not change. Taken together, the present results indicated that the antioxidantdefenses of M. borellii, mainly in hepatopancreas, were significantly affected by aquatic hydrocarboncontamination, regardless of the season.