INVESTIGADORES
GUEVARA Maria Gabriela
congresos y reuniones científicas
Título:
Improvement of StAP3 antimicrobial activity by PEGylation
Autor/es:
MUÑOZ; CARACCIOLO; DALEO; ABRAHAM; GUEVARA
Lugar:
Potrero de los Funes, San Luis
Reunión:
Simposio; Segundo Simposio Latinoamericano de Nanomedicinas; 2012
Institución organizadora:
Asociación Argentina de Nanomedicina
Resumen:
Potato aspartic proteases (StAPs) are proteins with tumoricidal and antimicrobial activities
which involve plasma membrane destabilization [1,2]. The StAPs selective
cytotoxic activity suggests that these proteins could be used to develop
alternative drugs with activity against microbes but not against mammalian
cells [3]. On the other hand, PEGylation
(i.e. the covalent link of PEG strands) is a technique used to improve
pharmaceutical properties of bioactive proteins, mainly improving the serum
half-life, and reducing the immunogenicity and the enzymatic degradation [4].
In order to evaluate the ability of PEGylation to improve StAPs antimicrobial activity, we have
studied the conjugation of mPEG-SVA (m.w. 5 kDa) to StAP3 under different conditions, and finally compared the cytotoxic activity of StAP3-PEG to that
of the native StAP3.