INVESTIGADORES
GIUSTO Norma Maria
artículos
Título:
High content of 22:6 (docosahexaenoate) and active (2-3H)glycerol metabolism of phosphatidic acid from photoreceptor membranes
Autor/es:
BAZÁN, N.; DI FAZIO DE ESCALANTE, M.S.; CAREAGA, M.; PASCUAL DE BAZÁN, H.E.; GIUSTO, N.M.
Revista:
BIOCHIMICA AND BIOPHYSICA ACTA
Editorial:
Elsevier Biomedical Press
Referencias:
Año: 1982 vol. 712 p. 702 - 705
ISSN:
0006-3002
Resumen:
This study describes the content, fatty acid composition and [2-3H]glycerol
metabolism of phosphatidic acid of rod outer segment membranes from
vertebrate retinas. A relatively high content of phosphatidic acid was
observed in rod outer segment membranes isolated from rat, toad and
bovine retinas. In bovine retinas, about 65% of the acyl groups of
phosphatidic acid were composed of docosahexaenoate. Arachidonate and
docosapentaenoate represented about 4 and 5%, respectively, of the
total, whereas stearate was the most common saturated acyl chain. An
active [2-3H]glycerol metabolism in the phosphatidic acid of
these membranes was found when whole retinas were incubated with the
precursor for short periods prior to subcellular fractionation. Our
results suggested that the pool of phosphatidic acid enriched in
docosahexaenoate may arise from de novo biosynthesis or from
phospholipid degradation by a phospholipase D enzyme, and that it is not
metabolically related, in any major fashion, to the diacylglycerols of
rod outer segment membranes.
