INVESTIGADORES
GIUSTO Norma Maria
artículos
Título:
Effects of aging on content, composition and synthesis of sphingomyeling in the central nervous system
Autor/es:
GIUSTO, N.M.; ROQUE, M.E.; ILINCHETA DE BOSCHERO, M.G.
Revista:
LIPIDS
Editorial:
SPRINGER HEIDELBERG
Referencias:
Año: 1992 vol. 27 p. 835 - 839
ISSN:
0024-4201
Resumen:
Sphingomyelin (SPH) content and composition in different regions of the
brain were analyzed in 2.5, 21.5 and 26.5-month-old rats. SPH content
increased in the cerebral hemispheres, cerebellum and medulla oblongata
plus pons as age increased. The highest SPH content was observed in
26.5-month-old rats, with values increasing by 1.74, 2.75 and 0.88-fold,
respectively, over 2.5-month-old rats. The SPH fatty acid composition
of brains from aged rats was markedly different from that of adult rats.
Between 2.5 and 26.5 months of age the monoenoic/saturated fatty acid
ratio increased from 0.22, 0.30 and 0.54 to 0.54, 0.68 and 1.03 in
cerebral hemispheres, cerebellum and medulla oblongata plus pons,
respectively. The percentage and content of fatty acids longer than 22
carbon atoms esterified to SPH increased with age from 18, 26 and 44 to
48, 52 and 62 mole % in cerebral hemispheres, cerebellum and medulla
oblongata plus pons in 26.5-month-old rats. In subcortical white matter
from aged rats, monoenoic 22-26 carbon atom fatty acids increased more
than the saturated ones in 21.5-month-old rats relative to 2.5-month-old
rats. In vitro synthesis of SPH from [3H]choline and [3H]palmitic acid
in cerebral cortex and cerebellum showed no significant differences
between adult rats and those 21.5 months of age. In cerebellum and in
cerebral cortex, [14C]serine incorporation increased in aged rats. The
results suggest that aging induces increases in both SPH content and in
the monoenoic/saturated fatty acid ratio. These increases are
quantitatively different in all brain regions analyzed.