EFFECT OF MATERNAL FRUCTOSE-RICH DIET (FRD) INTAKE ON THE ADULT OFFSPRING BROWNING POTENTIAL OF RETROPERITONEAL WHITE ADIPOSE TISSUE (RPWAT)

ALZAMENDI, ANA; MIGUEL IGNACIO; SPINEDI, EDUARDO; GIOVAMBATTISTA , ANDRÉS

Mar del Plata

Congreso; LXIV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2019

SAIC

Abstract/Resumen: Beige adipocytes dissipate energy as heatthrough uncoupling protein-1 (UCP1) activity. Cold exposure orß3-adrenergic agonist treatment stimulates white to beigeadipocyte conversion. Although not completely matched, UCP1expression levels are largely related to beige adipocytethermogenesis. Our aim was to assess whether maternal FRDintake throughout pregnancy affects the development ofbrowning capacity in RPWAT from the adult male offspring. Onpregnancy day 1, dams were provided with either tap water(control) or FRD (10 % w/v; in tap water) and fed ad libitumwith chow up to delivery. Weaned pups received water and chowad libitum up to 60 days of age (experimental day). C and Findicate pups born to control and FRD dams, respectively. Onday 53 of age, C and F pups were submitted to cold (4 ºC) forone week. Body weight and food intake was daily registered. Onexperimental day, trunk blood was collected and RPWAT padswere dissected and weighted. RPWAT mRNA expression levels ofbeige adipogenic markers were measured, and additional tissuewas used for H&E staining, IHC and Western blot analysis. Dataindicate that basal Pgc1a; and UCP1 expression were lower in Frats (p