COLD-INDUCED INGUINAL AND EPIDIDYMAL ADIPOSE TISSUES RESPONSES IN THE HYPERCORTICOSTERINEMIC MALE RAT

MARTIN FLORENCIA; SPINEDI, EDUARDO; GIOVAMBATTISTA , ANDRÉS

Mar del Plata

Congreso; LXIV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2019

SAIC

Abstract/Resumen: It is known that glucocorticoids (GC) have different effects on white adipose tissue (WAT) and inhibit brown adipose tissue (BAT) thermogenesis; however, it remains unclear how GC affect the thermogenic process in different WAT depots. Our aim was to study, in a hypercorticosteronemic rat model, the thermogenic activity in subcutaneous inguinal and epididymal WAT pads (scWAT and eWAT, respectively). For this aim, male SDrats neonatally-treated with monosodium L-glutamate (MSG) were used. A subgroup of prepubertal 30 day-old male CTR and MSG rats were exposed to cold (4 °C) during 7 days. MSG and litter-mate CTR scWAT and eWAT pads were dissected, weighed and processed for mRNA (qPCR) and protein (Western blot) analysis. Data were analyzed by Two-way ANOVA. Body weight gain was reduced by MSG treatment (p