Maternal Fructose Intake during Gestation Inhibits Male Offspring?s Adipogenic Potential of White Adipose Tissue Precursor Cells

ALZAMENDI, ANA; GAMBARO SABRINA ELIANA; MIGUEL IGNACIO; SPINEDI, EDUARDO; GIOVAMBATTISTA , ANDRÉS

Orlando

Congreso; Annual Meeting 2017 Endocrine Society; 2017

Endocrine Society

Previous studies from our group indicate that fructose rich diet (FRD) consumption during gestation induces a decrease in adipose precursor cells (APCs) from retroperitoneal adipose tissue (RPAT). This was accompanied by a reduced RPAT mass in the first adult male progeny. These changes favor for adipocyte hypertrophy and distorted pattern of leptin secretion. We now evaluated the impact of FRD intake by the gestating dams on the adipogenic capacity of stromal vascular fraction (SVF) cells from RPAT of their first adult male progeny. On pregnancy day 1, dams were provided with either tap water alone (control) or containing fructose (10% w/v in drinking water; FRD) and fed ad libitum with chow, up to delivery. Lactating dams and their pups (between 21 and 60 of age) received water and chow ad libitum. C and F animals were born to control and FRD dams, respectively. At adult life (age 60 days) male rats were sacrificed and RPAT pads were dissected, then SVF cells were isolated. Trunk blood was collected to corroborate hyperleptinemia. The mRNA expression levels of adipogenic potential markers were assessed by qPCR in RPAT SVF cells, in relation to β-actin and 18S RNA expression. PPARγ expression was quantified in differentiating cells, by immunofluorescence. Terminal differentiation was evaluated by Papanicolau staining and expression markers by qPCR. Also, RPAT expression of functional markers from whole tissue was assessed. Our data indicated that RPAT SVF cells from F rats expressed lower and higher (p