MATERNAL ADVERSE DIET AND WHITE ADIPOSE TISSUE BROWNING CAPACITY OF ADULT OFFSPRING.

ALZAMENDI, ANA; BOZZANO ALEJANDRO; REY AMANDA; SPINEDI, EDUARDO; GIOVAMBATTISTA , ANDRÉS

Mar del Plata

Congreso; LXVI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA; 2022

SAIC

Beige adipocytes dissipate energy as heat through uncoupling protein-1 (UCP1) activity. Our aim was to assess whether maternal fructose rich diet (FRD) intake during lactation affects development of browning capacity of retroperitoneal white adipose tissue (WRPAT) from adult male offspring. Adult female rats were mated, at birth pups were counted and they were equal to 8 per mother. Dams were provided with either tap water (control) or FRD (10 % w/v; in tap water) and fed ad libitum with chow up to day 21 of birth. Weaned pups received water and chow ad libitum up to 60 days of age (experimental day). C and F indicate pups born to control and FRD dams, respectively. At the age of 53 days, C and F pups were kept at 4ºC for one week. Body weight and food intake was registered every day. On experimental day, trunk blood was collected, WRPAT pad was dissected and weighted. mRNA expression levels of beige adipogenic markers were assessed in WRPAT pads, and additional tissue was used for H&E staining and IHC. In basal conditions, F animals gain more body weight as they consumed more calories, and moreover, ucp1 WRPAT expression was significantly higher in F than C animals. Cold exposure induced, in both groups, an increase in BAT mass, a decrease in body weight, in WRPAT mass, and in circulating triglycerides levels. Moreover, when submitted to cold F animals increased ucp-1 expression above C cold animals. And this increase was consistent with H&E stain showing beige areas in WRPAT, and UCP1 positive cells by IHC. This ucp1 gene expression pattern was accompanied by a higher pgc-1α expression in F cold animals, significantly higher than all other groups. Our results support the ability to mount a differential response to low temperatures by the animal in its adult stage, when it is subjected to metabolic programming by administration of a diet rich in fructose to the mother, in the infant stage, probably due to a compensatory mechanism (PICT2020-3064).