INVESTIGADORES
GENTI Susana
artículos
Título:
Carbonyl reduction of insecticide of an anti-inset agent imidazole analogue of metyrapone in soil bacteria, invertebrate and vertebrate species
Autor/es:
OPPERMANN UDO; NAGEL GERD; BELAI IVAN; BUELD JULIANE; GENTI DE RAIMONDI SUSANA; KOOLMAN JAN; NETTER KARL; MASER EDMUND
Revista:
CHEMICO-BIOLOGICAL INTERACTIONS
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Año: 1998 vol. 114 p. 211 - 224
ISSN:
0009-2797
Resumen:
Carbonyl reduction to the respective alcohol metabolites of the anti-insect agent imidazole
analogue of metyrapone, NKI 42255 (2-(1-imidazolyl)-1-(4-methoxyphenyl)-2-methyl-1-
propanone) and its parent compound metyrapone was characterized in subcellular fractions
previously described bacterial and mammalian hydroxysteroid dehydrogenases:carbonyl
from soil bacteria, as well as insect, invertebrate and teleost species. The enzymes involved
in this metabolic step were characterized with respect to their cosubstrate specificities,
inhibitor susceptibilities, and immunological crossreactivities with antibodies directed against reductases (HSD:CR). All fractions investigated rapidly reduced metyrapone, with highest
specific activities found in insect, invertebrate and vertebrate fractions. Except for the insect
fractions, all species examined reduced the NKI compound. Cosubstrate dependence and
inhibitor specificities suggest that the enzymes described belong to the protein superfamilies
of short-chain dehydrogenases:reductases (SDR) or aldo-keto reductases (AKR). Immunological
crossreactions to the previously established subgroup of HSD:CRs were found in
trout liver microsomes and insect homogenates, but not in all bacterial extracts or earthworm
microsomes. These findings suggest that the high CR activities found in these fractions
belong to different subgroups of SDR or AKR. © 1998 Elsevier Science Ireland Ltd. All
rights reserved.