INVESTIGADORES
GENTI Susana
artículos
Título:
Identification of multiple differentially expressed messenger RNAs in normal and pathological trophoblast
Autor/es:
DURAND SANDRA; ABADIE PAULA; ANGELETTI SOFÍA; GENTI DE RAIMONDI SUSANA
Revista:
PLACENTA
Editorial:
W B SAUNDERS CO LTD
Referencias:
Lugar: Londres; Año: 2003 vol. 24 p. 209 - 214
ISSN:
0143-4004
Resumen:
In an attempt to assess the molecular basis of phenotypic alterations present in the gestational trophoblastic diseases (GTDs) and
to identify genes whose expression is specifically associated to these placental proliferative disorders we performed differential
display techniques. Initially 19 candidate gene fragments were identified and differential expression was confirmed in eight of these
fragments by Northern blot analysis. At the mRNA level ribosomal L26 (rL26), ribosomal L27 (rL27), a new Kru¨ppel type zinc
finger protein and TIS11d were preferentially expressed in normal early placenta (NEP) relative to complete hydatidiform mole
(CHM), persistent gestational trophoblastic disease (PGTD) and choriocarcinoma JEG-3 cell line. In contrast, heterogeneous
ribonucleoprotein A1 (hnRNPA1), the ferritin light chain mRNA, and the uncharacterized protein KIAA0992 were predominantly
expressed in JEG-3 cell line. Finally, decorin, a prototype member of an expanding family of small leucine-rich proteoglycans,
showed high expression in CHM. In addition we demonstrated by immunohistochemistry analysis that increased decorin mRNA
in CHM reflected a genuine augmentation in average steady state mRNA levels within cells. Taken together, these findings
provide several interesting candidates for regulation of tumorigenic expression as well as early placentation development, including
those involved in protein synthesis (rL26 and rL27), metabolism (ferritin light chain), intercellular communication (decorin) and
regulation of gene expression (Kruppel-like zinc finger, TIS11d and hnRNPA1). Information about such alterations in gene
expression could be useful for elucidating the genetic events associated to gestational trophoblastic pathogenesis, developing new
diagnostic markers, or determining novel therapeutic targets.