INVESTIGADORES
GARDENAL Cristina Noemi
artículos
Título:
Two different routes of colonization of Aedes aegypti in Argentina from neighboring countries.
Autor/es:
RONDAN DUEÑAS, J.C., ALBRIEU LLINÁS, G., PANZETTA-DUTARI, G.M. Y GARDENAL, C.N.
Revista:
JOURNAL OF MEDICAL ENTOMOLOGY
Editorial:
ENTOMOLOGICAL SOC AMER
Referencias:
Año: 2009 vol. 46 p. 1344 - 1354
ISSN:
0022-2585
Resumen:
ABSTRACT Aedes aegypti L. (Diptera, Culicidae) is the main vector of dengue and yellow fever.
In Argentina, the species was apparently eradicated approximately in 1964; by 1986, it was reintroduced.
To identify different gene pools in geographical populations of the species and to ascertain the
possible routes of colonization, we analyzed the diversity of mitochondrial DNA haplotypes in 572
specimens from Argentina and neighboring countries. We found that the restriction fragment length
polymorphism-polymerase chain reaction screening of a large DNA fragment including the AT-rich
region was the best strategy to reconstruct the colonization pattern of Ae. aegypti in Argentina. Twenty
haplotypes were recognized; levels of genetic similarity varied among populations from different
geographical locations. The haplotype network constructed on the basis of genetic distances showed
three well differentiated groups. Two of them exhibited a well deÞned spatial distribution and
populations in these groups presented an isolation-by-distance pattern. The persistence of relictual
populations after the last eradication campaigns would explain the high levels of haplotype diversity
and the presence of exclusive haplotypes in urban centers from northwestern Argentina. Eastern
Argentine populations showed one prevalent haplotype, also predominant in Brazil and Paraguay. Our
results highlight the need for efÞcient surveys and control campaigns, given the strong effect of land
trade on genetic exchange among mosquito populations from Argentina and neighboring countries
where dengue is endemic.
haplotypes were recognized; levels of genetic similarity varied among populations from different
geographical locations. The haplotype network constructed on the basis of genetic distances showed
three well differentiated groups. Two of them exhibited a well deÞned spatial distribution and
populations in these groups presented an isolation-by-distance pattern. The persistence of relictual
populations after the last eradication campaigns would explain the high levels of haplotype diversity
and the presence of exclusive haplotypes in urban centers from northwestern Argentina. Eastern
Argentine populations showed one prevalent haplotype, also predominant in Brazil and Paraguay. Our
results highlight the need for efÞcient surveys and control campaigns, given the strong effect of land
trade on genetic exchange among mosquito populations from Argentina and neighboring countries
where dengue is endemic.
In Argentina, the species was apparently eradicated approximately in 1964; by 1986, it was reintroduced.
To identify different gene pools in geographical populations of the species and to ascertain the
possible routes of colonization, we analyzed the diversity of mitochondrial DNA haplotypes in 572
specimens from Argentina and neighboring countries. We found that the restriction fragment length
polymorphism-polymerase chain reaction screening of a large DNA fragment including the AT-rich
region was the best strategy to reconstruct the colonization pattern of Ae. aegypti in Argentina. Twenty
haplotypes were recognized; levels of genetic similarity varied among populations from different
geographical locations. The haplotype network constructed on the basis of genetic distances showed
three well differentiated groups. Two of them exhibited a well deÞned spatial distribution and
populations in these groups presented an isolation-by-distance pattern. The persistence of relictual
populations after the last eradication campaigns would explain the high levels of haplotype diversity
and the presence of exclusive haplotypes in urban centers from northwestern Argentina. Eastern
Argentine populations showed one prevalent haplotype, also predominant in Brazil and Paraguay. Our
results highlight the need for efÞcient surveys and control campaigns, given the strong effect of land
trade on genetic exchange among mosquito populations from Argentina and neighboring countries
where dengue is endemic.
haplotypes were recognized; levels of genetic similarity varied among populations from different
geographical locations. The haplotype network constructed on the basis of genetic distances showed
three well differentiated groups. Two of them exhibited a well deÞned spatial distribution and
populations in these groups presented an isolation-by-distance pattern. The persistence of relictual
populations after the last eradication campaigns would explain the high levels of haplotype diversity
and the presence of exclusive haplotypes in urban centers from northwestern Argentina. Eastern
Argentine populations showed one prevalent haplotype, also predominant in Brazil and Paraguay. Our
results highlight the need for efÞcient surveys and control campaigns, given the strong effect of land
trade on genetic exchange among mosquito populations from Argentina and neighboring countries
where dengue is endemic.
Aedes aegypti L. (Diptera, Culicidae) is the main vector of dengue and yellow fever.
In Argentina, the species was apparently eradicated approximately in 1964; by 1986, it was reintroduced.
To identify different gene pools in geographical populations of the species and to ascertain the
possible routes of colonization, we analyzed the diversity of mitochondrial DNA haplotypes in 572
specimens from Argentina and neighboring countries. We found that the restriction fragment length
polymorphism-polymerase chain reaction screening of a large DNA fragment including the AT-rich
region was the best strategy to reconstruct the colonization pattern of Ae. aegypti in Argentina. Twenty
haplotypes were recognized; levels of genetic similarity varied among populations from different
geographical locations. The haplotype network constructed on the basis of genetic distances showed
three well differentiated groups. Two of them exhibited a well deÞned spatial distribution and
populations in these groups presented an isolation-by-distance pattern. The persistence of relictual
populations after the last eradication campaigns would explain the high levels of haplotype diversity
and the presence of exclusive haplotypes in urban centers from northwestern Argentina. Eastern
Argentine populations showed one prevalent haplotype, also predominant in Brazil and Paraguay. Our
results highlight the need for efÞcient surveys and control campaigns, given the strong effect of land
trade on genetic exchange among mosquito populations from Argentina and neighboring countries
where dengue is endemic.
haplotypes were recognized; levels of genetic similarity varied among populations from different
geographical locations. The haplotype network constructed on the basis of genetic distances showed
three well differentiated groups. Two of them exhibited a well deÞned spatial distribution and
populations in these groups presented an isolation-by-distance pattern. The persistence of relictual
populations after the last eradication campaigns would explain the high levels of haplotype diversity
and the presence of exclusive haplotypes in urban centers from northwestern Argentina. Eastern
Argentine populations showed one prevalent haplotype, also predominant in Brazil and Paraguay. Our
results highlight the need for efÞcient surveys and control campaigns, given the strong effect of land
trade on genetic exchange among mosquito populations from Argentina and neighboring countries
where dengue is endemic.
Ae. aegypti in Argentina. Twenty
haplotypes were recognized; levels of genetic similarity varied among populations from different
geographical locations. The haplotype network constructed on the basis of genetic distances showed
three well differentiated groups. Two of them exhibited a well deÞned spatial distribution and
populations in these groups presented an isolation-by-distance pattern. The persistence of relictual
populations after the last eradication campaigns would explain the high levels of haplotype diversity
and the presence of exclusive haplotypes in urban centers from northwestern Argentina. Eastern
Argentine populations showed one prevalent haplotype, also predominant in Brazil and Paraguay. Our
results highlight the need for efÞcient surveys and control campaigns, given the strong effect of land
trade on genetic exchange among mosquito populations from Argentina and neighboring countries
where dengue is endemic.