Phospholipase C role in stomatal development

IGNACIO BERAMENDI; DENISE SCUFFI; ANA MARÍA LAXALT; CARLOS GARCIA-MATA

Santa Fe

Congreso; Reunión Argentina de Fisiología Vegetal; 2021

SAFV

Guard cells (GC) are in charge of gas exchange and transpiration in plants through the formation and regulation of stomatal pore. Stomata are formed by successive divisions from undifferentiated meristemoid cells, passing through the stage of meristemoid cells (M) and guard mother cells (GMC). Phosphoinositide-specific phospholipases C (PI-PLC) is a 9 member family genes in Arabidopsis (PLC1-PLC9) . These enzymes regulate development and environmental responsesIn Arabidopsis PLC2, 3, 5 and 7 participate in the regulation of stomatal closure, but the role of PLC enzymes in stomatal development is still unknown.  To evaluate the role of PLC 5 during stomatal development we analyze M, GCM and GC indices and densities, in wild type (wt), mutant (plc5, Knockdown), and PLC5 overexpressing (OEPLC5) 7-days old Arabidopsis plants. We found that OEPLC5 have a higher GC index and stomatal density compare with wt andplc5 plants. On the other hand, no differences were found among plants for M and GMC indices indicating that PLC5 has a role in final steps. Additionally, we are studying the expression pattern of PLC5 in the different developmental stages, and whether this pattern is correlated with the expression of well-known key transcriptional factors.