Hydrogen Sulfide Increases Production of NADPH Oxidase-Dependent Hydrogen Peroxide and Phospholipase D-Derived Phosphatidic Acid in Guard Cell Signaling

SCUFFI, DENISE; NIETZEL, THOMAS; DI FINO, LUCIANO M.; MEYER, ANDREAS J.; LAMATTINA, LORENZO; SCHWARZLÄNDER, MARKUS; LAXALT, ANA M.; GARCÍA-MATA, CARLOS

PLANT PHYSIOLOGY.

AMER SOC PLANT BIOLOGISTS

Año: 2018 vol. 176 p. 2532 - 2542

0032-0889

Hydrogen sulfide (H2S) has emerged as an important gaseous signaling molecule in plants participating in several stresses and developmental processes. L-cysteine desulfhydrase 1 (DES1), one of the enzymatic sources of H2S in plants, was shown to participate in ABA-induced stomatal closure. In this work we combined pharmacological and genetic approaches to elucidate the involvement of H2S in stomatal closure and the interplay between H2S and other second messengers of the guard cell signaling network, such as hydrogen peroxide (H2O2) and phospholipase D (PLD)-derived phosphatidic acid (PA) in Arabidopsis. Both NADPH oxidase isoforms, RBOHD and RBOHF, were required for H2S-induced stomatal closure. H2S stimulated H2O2 production in Arabidopsis guard cells, as assessed by in vivo imaging using the cytosolic ratiometric fluorescent biosensor roGFP2-Orp1. We further found interplay between H2S and PLD activity in the regulation of ROS production and stomatal movement. PLDα1 and PLDδ isoforms were required for H2S-induced stomatal closure. Yet, most of H2S-dependent H2O2 production requires the activity of PLDα1. Finally we show that H2S induced PLDδ-derived PA levels in guard cells. Together our data demonstrate an involvement of H2S in the signaling network that controls stomatal closure, and suggest H2S as a new regulator of NADPH oxidase and PLD activity in guard cell.