The complete nucleotide sequence of a Spanish isolate of Citrus psorosis virus: comparative analysis with other ophioviruses.

SUSANA MARTÍN, CARMELO RINCÓN, MARÍA LAURA GARCÍA, GABRIELA NAUM-ONGANÍA, OSCAR GRAU, RICARDO FLORES, PEDRO MORENO AND JOSÉ GUERRI

ARCHIVES OF VIROLOGY

Año: 2005 vol. 150 p. 167 - 176

0304-8608

The complete nucleotide sequence of Citrus psorosis virus  (CPsV), isolate P-121 from Spain, was determined and compared with known sequences from isolate CPV-4 and from other ophioviruses. The three RNAs of P-121 had similar size and identical organization as those of CPV-4. The 24K and the RdRp proteins encoded in the viral complementary (vc) strand of the P-121 RNA1 showed 86.9% and  91.6% amino acid identity, respectively, with those of the CPV-4 isolate, but only 22.4% and 37.7% with homologous proteins in Mirafiori lettuce virus (MiLV), the other ophiovirus species completely sequenced. Identities between the vcRNA2-encoded 54K protein, and the vcRNA3-encoded coat protein, of CPsV isolates P-121 and CPV-4 were 92.8% and 94.1%, respectively, whereas identities with homologous proteins of MiLV were 24.9% and 33.3%. MiLV was closer to other ophioviruses than to CPsV. Biological and genetic differences between CPsV and the other ophioviruses support their allocation in different genera.