INVESTIGADORES
GARCIA Maria Laura
artículos
Título:
Genetic variation of populations of Citrus psorosis virus, a negative stranded RNA plant virus.
Autor/es:
MARTÍN S, GARCÍA ML, TROISI A, RUBIO L, LEGARRETA G, GRAU O, ALIOTO D, MORENO P, GUERRIJ
Revista:
JOURNAL OF GENERAL VIROLOGY
Referencias:
Año: 2006 vol. 87 p. 3097 - 3102
ISSN:
0022-1317
Resumen:
Citrus psorosis virus (CPsV), the type species of genus Ophiovirus, has
a segmented, negative-stranded RNA genome. We examined the population
structure and genetic variation of CPsV in three coding regions located
in RNAs 1, 2 and 3, analysing 22 isolates from Argentina, California,
Florida, Italy and Spain. Most isolates contained a predominant
sequence and some minor variants. Estimations of the genetic diversity
and phylogenetic clustering of isolates disclosed two populations, one
comprising isolates from Spain, Italy, Florida and California and the
other including the Argentinean isolates. Isolate CPV-4 (from Texas)
included for comparison was distant from both groups, suggesting that
it belongs to a third group. The low ratio between non-synonymous and
synonymous nucleotide substitutions indicated strong selection for
amino acid sequence conservation, particularly in the coat protein
gene. Incongruent phylogenetic relationships in different genomic
regions suggested that exchange of genomic segments may have
contributed to CPsV evolution.