RNA 2 of Citrus psorosis virus is of negative polarity and has a

MARIA EUGENIA SANCHEZ DE LA TORRE, CARMELO LOPEZ, OSCAR GRAU AND MARı!A LAURA GARCIA

JOURNAL OF GENERAL VIROLOGY

SOC GENERAL MICROBIOLOGY

Lugar: London; Año: 2002 p. 1777 - 1781

0022-1317

Citrus psorosis virus (CPsV) causes a citrus diseaseoccurring worldwide. Isolate CPV 4 has a genomewith three single-stranded RNAs. The completesequence of RNA 2 (1643 nucleotides) is reportedhere. Northern blot hybridization with strandspecificprobes showed that most of the encapsidatedRNA 2 is of negative polarity, although asmall amount of the complementary strand mayalso be present in particles. The RNA 2 complementarystrand contained a single open readingframe encoding a protein of 476 amino acids, whichincludes a motif resembling a nuclear localizationsignal. The sequence of this putative protein showsno significant similarity to any other in the databases.In the 3«-terminal untranslated region thereis a putative polyadenylation signal. No subgenomicRNAs derived from RNA 2 were detected.