INVESTIGADORES
GAMARNIK Andrea Vanesa
artículos
Título:
A 5 RNA Element Promotes Dengue Virus RNA Synthesis on a Circular Genome
Autor/es:
CLAUDIA FILOMATORI; MARIA LODEIRO; DIEGO ALVAREZ; MARCELO SAMSA; LÍA PIETRASANTA; ANDREA GAMARNIK
Revista:
GENES & DEVELOPMENT.
Referencias:
Año: 2006 p. 2238 - 2249
ISSN:
0890-9369
Resumen:
The mechanisms of RNA replication of plus strand RNA viruses are still unclear. Here, we identified the first promoter element for RNA synthesis described in a flavivirus. Using dengue virus as a model, we found that the viral RdRp discriminates the viral RNA by specific recognition of a 5 element named SLA. We demonstrated that RNA-RNA interactions between 5 and 3 end sequences of the viral genome enhance dengue virus RNA synthesis only in the presence of an intact SLA. We propose a novel mechanism for minus strand RNA synthesis in which the viral polymerase binds SLA at the 5 end of the genome and reaches the site of initiation at the 3 end via long-range RNA-RNA interactions. These findings provide an explanation for the strict requirement of DV genome cyclization during viral replication