INVESTIGADORES
FURLAN Ricardo Luis Eugenio
capítulos de libros
Título:
Enzymatic Bioautographic Methods
Autor/es:
RAMALLO, IA; SALAZAR, MO; FURLAN, RLE
Libro:
Targeting Enzymes for Pharmaceutical Development: Methods and Protocols
Editorial:
Springer Science & Business Media LLC
Referencias:
Lugar: New York; Año: 2019; p. 179 - 189
Resumen:
Enzymatic bioautography enables the detection of enzyme inhibitors absorbed on a thin-layer chromatography plate. Therefore, it is an assay format that is particularly useful for the detection of inhibitors present in complex mixtures. The inhibition properties of compounds separated by thin-layer chromatography can be directly analyzed to produce an inhibition profile. Here, we describe the conditions to detect inhibitor of the enzymes xanthine oxidase and β-glucosidase immobilized on agar gel.
