INVESTIGADORES
FURLAN Ricardo Luis Eugenio
artículos
Título:
A Rapid TLC Autographic Method for the Detection of Xanthine Oxidase Inhibitors and Superoxide Scavengers
Autor/es:
RAMALLO IA; ZACCHINO SA; FURLAN RLE
Revista:
PHYTOCHEMICAL ANALYSIS
Editorial:
John Wiley & Sons- Ltd.
Referencias:
Lugar: Chichester, Sussex, UK; Año: 2006 vol. 17 p. 15 - 19
ISSN:
0958-0344
Resumen:
A new bioautographic assay suitable for the localization of xanthine oxidase inhibitors and superoxide radical scavengers present in a complex matrix is described. Enzyme activity is detected by reaction of superoxide radicals with nitroblue tetrazolium to form a blue formazan salt. Both activities can be differentiated using a non-enzymatic version of the autographic assay wherein superoxide is chemically generated
