INITIAL CHARACTERIZATION OF GLUTAMATE RECEPTOR IN MOUSE SPERM

SORIA M. SELTZER A Y FORNÉS M.; SELTZER A.; FORNES MW.

Merlo, San Luis

Congreso; XXII Reunión Anual de la Sociedad de Biol de Cuyo; 2021

SBCUYO

Glutamic acid (GA)is well-known excitatory neurotransmitters. Recentrly was involved in triggering the Arosomal Reaction in mouse and human sperm. More over the signal pathway involved in this novel signal transduction system was characterized. But the receptor/s involved in sperm are not characerized to this moment. In this way, two receptors (Glu-R), Ionotropics (AMPA/Kainico, NMDA) and Metabotropics, had been described at the CNS. Using western blot and immune cytochemistry methods we try to identify Glu-R in mouse sperm. The following Antibodies were used. Anti metabotropic-mGluR2/3 (mGluR2 and mGluR3) and mGluR5/1 (mGluR5 and mGluR1a) - and NMDA - pan-NMDA (NR1, for splice variants) and NMDAR-2C (NMDAR-2A and NMDAR-2B). Homogenates from cerebral cortex (CC), hipocampus (Hipo) (as controls) and mouse sperm were used as Glu-R source. Two proteins bands in sperm homogenates were detected with anti mGlu-R NMDAR2C, that were coincident with CC and Hipo; and two bands with anti mGluR 5/1 also expressed in CC and Hipo. Subtype NMDAR 1 was detected in CC but not in sperm. Inmuno cyto chemistry techniques permit to locate NMDAR-2C at the dorsal edge of sperm head and mGlu-R over the acrosomal region. More effort must be done to identify the Glu-R but subtypes of them were identifying here by two independent methods. Results presented here support the idea that GA could trigger the acrosomal reaction in mouse sperm.