HIGH FAT DIETS INCREASE METHYLGLYOXAL BLOOD LEVELS WHICH ARE REDUCED BY OLIVE OIL INTAKE

SIMÓN LAYLA; FUNES A; COLOMBO REGINA; SAEZ LANCELOTTI E; MONCLUS M A; CABRILLANA ME; BARBIERI MA; FORNES MW.

Mendoza

Congreso; Reunion anual de la sociedad biologia de cuyo; 2016

Soc. de Biol. de Cuyo

A143HIGH FAT DIETS INCREASE METHYLGLYOXAL BLOOD LEVELS WHICH ARE REDUCED BY OLIVE OIL INTAKESimón L1, Funes A1, Colombo R1, Saez E1, Monclus M1, Cabrillana E1, Barbieri A2, Fornés M1.1 IHEM, UNCUYO, CONICET, Mendoza, Argentina. 2 FIU, Florida, Miami. lsimonujam@gmail.com MethylGlyoxal (MG) is a glycation end product found in patients with diabetes, a Chronic Adult Disease (CAD). Another CAD is HyperCholesterolemia (HC). A classic animal model to study effects of HC is rabbit fed with high fat diets (HCR). Also HC can be reverse by the addition of Olive Oil (OO) to diets. The purpose of this study was to determinate MG variations under high fat and OO intake. To generate HCR, rabbits were fed with balanced diet supplemented with grease. After 6 months, fat was reduced and replaced by OO (1:1, fat:OO). Blood samples were analyzed by several biochemical parameters. In HCR, cholesterolemia increased three fold (87 ± 12 mg/dl) over control (25 ± 4 mg/dl, p < 0,001). The addition of OO to diet promoted a cholesterol lowering (48 ± 5 mg/dl). Curiously, triglyceridemia and glycemia showed a non-significant increment. MG blood levels increased in HCR (Δ=385 μg/dl compared to NCR Δ=96 μg/dl; Δ = differences between 6 months of treatment and 0 month) and decreased in OO rabbits (Δ=-485 μg/dl). MG normally linked to glycemia could be also related to cholesterolemia. OO may benefit the HCR group by preventing the formation of MG.