NOVEL FINDING OF A TESTICULAR PROTEIN IN KIDNEY DUCTS

CABRILLANA ME; MONCLUS MA; SIMON LAYLA; FUNES A ; COLOMBO REGINA; SAEZ LANCELLOTTI E; VINCENTI AE; FORNES MW.

Mendoza

Congreso; Reunión Anual de la Sociedad de Biología de Cuyo; 2016

Soc. de Biol. de Cuyo

A68NOVEL FINDING OF A TESTICULAR PROTEIN IN KIDNEY DUCTSCabrillana ME1,2, Monclus MA1,2, Simon L1, Funes A1, Colombo R1, Saez L1,2, Vincenti AE1 and Fornés MW1,2.1Instituto de Histología y Embriología (UNCuyo-CONICET), Mendoza. 2Consejo de Investigaciones de la Universidad del Aconcagua (CIUDA, Mendoza, Argentina. Email: mecabrillana@yahoo.com.arDuring sperm maturation, tail structures are stabilized, through oxidation of thiol groups. It was proposed that sperm thiol oxidation is necessary for sperm motility. This oxidation occurs in many proteins of the sperm, like ODF1. ODF1 is a cytoskeleton protein which function in the sperm has not been clarified at all. ODF1 was described as a testis and sperm protein exclusively, and was involved in the development of the flagellum. The aim of this work was to evaluate the presence of this protein in other tissues. We applied western blot, RT-PCR, and inmunofluorescence techniques using an antibody against ODF1. Surprisingly, the strongest signal was found in the proteins obtained from marrow kidney and cells from collecting duct. Also some cortical ducts presented positive mark. Extracts from brain, liver, skin, and lung did not show positive signal to ODF1 antibody. By RT-PCR we identified the mARN of this protein in kidney and testis extracts. This was the first time that ODF1 was described in other tissue. In the future we will analyze if ODF1 participates in the development of the primary cilium in collecting ducts.