GLUTAMIC ACID: LOCATION AND FUNCTIONAL STUDIES IN SPERM – SPERMATOGENESIS - AND FALLOPIAN TUBE IN MOUSE.

SORIA M; GIULIANI F; CABRERA R; FORNES M W

Caja de los Trebejos - Potrero de los Funes. SAN LUIS.

Congreso; XXIV REUNIÓN CIENTÍFICA ANUAL DE LA SOCIEDAD DE BIOLOGÍA DE CUYO Y IV REUNIÓN CIENTÍFICA DE LA SOCIEDAD ARGENTINA DE MICROSCOPIA (SAMIC).; 2006

Sociedad de Biología de Cuyo

Glutamic Acid in sperm, fallopian tube and nervous central system. Soria MG, Giuliani FA, Cabrera RJ y Fornés MW. msoria@fcm.uncu.edu.ar IHEM - Facultad de Ciencias Médicas - UNCuyo. Mendoza. CP 5500 The Glutamic Acid (Glu) is a excitatory neurotransmitter that uses receptors (Rc) of two types: Ionotropics (AMPA/Kainic Acid, NMDA) and Metabotropics (mGluRs). They are well study at the central nervous system (CNS), although these receptors have not been fully described in sperms or fallopian tubes. We have reported GluRc in sperm cell and induction of acrosomal reaction (AR). In concentrations µmolares Glu promotes the acrosomal reaction. Specific antibodies anti mGluR2/3, mGluR5/1 and anti-Pan NMDA-NR1 and NMDAR-2A, 2B and 2C localize these GluRc in sperms and mouse´s testis. Our studies find differences in the patterns of staining of the cells, with the exception of NMDA-NR1, which gave negative. The stain was observed in acrosome and tail, principal portion according which the antibody used.  But major stain was detected with antibody against mGlu2/3. Some of these antibodies were measured for inmunoblotting in homogenates of mouse espermatozoa, and in homogenates of brain cortex and hippocampus of rats (control). Bands were detected in sperms coinciding with the brain counterparts. After that, it was demonstrated the effect of the Glu on the acrosomal reaction by means of fluorescence technique. More recently, superfusion assays were performed to determine the release and uptake of Glu in the uterine horns of mice females, because of it is this place where the AR takes place after ejaculation. These experiments show that fallopian tube could release-uptake Glu. Moreover, these interchange had relationship with the stadium of the female sexual cycle. The biggest uptake was observed during the diestrus, with significant differences between this stadium and the metaestrus and the estrus. The release of Glu was major in the estrus, with significant difference between this one and the diestrus and metaestrus. Overall results indicate that sperm posses GluRc, Glu promote AR and Glu is present at the site of AR during the fertilization moment.