INVESTIGADORES
FERNANDEZ IRIARTE Pedro Jose
artículos
Título:
First identification of Nosema ceranae (Microsporidia) infecting Africanized Honeybees in Venezuela
Autor/es:
PORRINI, L; M.P. PORRINI, ; P. M. GARRIDO; J. PRINCIPAL,; C. BARRIOS; B. BIANCHI; FERNÁNDEZ IRIARTE P.; EGUARAS M
Revista:
JOURNAL OF APICULTURAL SCIENCE
Editorial:
RESEARCH INST POMOLOGY FLORICULTURE
Referencias:
Año: 2017
ISSN:
1643-4439
Resumen:
Nosema ceranae is a pathogen of Apis mellifera, which seems to have jumped from its original host Apis ceranae. Nosemosis affecting the honey bee Apis mellifera is caused by two parasitic fungi described as etiologic agents of the disease. Nosema apis was the only microsporidian infection described in A. mellifera until N. ceranae was identified in Taiwan and Europe. Nosema spp. positive samples of adult worker bees from Lara state, Venezuela, were determined by light microscopy of spores. Then, samples were tested to determine species using previously reported PCR primers for the 16S rRNA gene. Due it is difficult to distinguish N. apis and N. ceranae morphologically; a Multiplex PCR assay has been used to differentiate both species. Only N. ceranae was found in analyzed samples and percentage of infected foragers fluctuated between 18% and 60%.
