New approaches for the determination of benzodiazepines using fluorescence quenching

GUIZZADO, VIRGINIA; FERNÁNDEZ, LUCIANA; PIRCHIO, PAULA; ACOSTA, GIMENA; FERNANDEZ, LILIANA; PERALTA, CECILIA

Córdoba (virtual)

Congreso; VI Reunión Internacional de Ciencias Farmacéuticas; 2021

RiCiFa

In recent years, ingredients in pharmaceutical formulations have received great attention as possible bioactive chemicals that are continuously introduced into the environment, often in small concentrations, affecting water quality and the environment. Benzodiazepines (BZPs) are widely consumed by modern society as minor tranquilizers, anxiolytics, hypnotics. According to data provided by the Argentine Pharmaceutical Confederation, Argentina shows a growing consumption of psychotropic drugs for more than a decade, a situation that has deepened in the last year as a result of social and psychological factors linked to the pandemic. The objective of this work is to propose a strategy for the indirect determination of three BDZs (alprazolam, clonazepam and diazepam) by means of the attenuation that they generate in the fluorescent signal (quenching effect) of the serum albumin molecule bovine (BSA). Different experimental parameters that influence the quenching effect of BDZs on BSA were studied (λex = 280 nm, λem = 340 nm, slits: 5/5). Also, the implemented strategies will be incorporated into schemes that combine high resolution liquid chromatography associated with fluorescent detection in order to partially or totally automate the methodology and allow a simultaneous determination of the aforementioned BDZs in ambiental samples.