INVESTIGADORES
FERNANDEZ Liliana Patricia
artículos
Título:
A NOVEL APPLICATION OF IMMOBILIZATION ON MEMBRANES FOR THE SEPARATION AND SPECTROFLUORIMETRIC QUANTIFICATION OF AMILORIDE AND FUROSEMIDE IN PHARMACEUTICAL SAMPLES
Autor/es:
PERALTA, CECILIA; FERNANDEZ, LILIANA; MASI, ADRIANA
Revista:
ANALYTICA CHIMICA ACTA
Editorial:
Elsevier
Referencias:
Año: 2010 vol. 661 p. 85 - 90
ISSN:
0003-2670
Resumen:
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A new, simple and highly sensitive
method for spectrofluorimetric determination of amiloride (AMI) and
furosemide (FUR) in pharmaceuticals is
presented. The proposed method is based on the separation of
AMI from FUR by solid-phase extraction
using a nylon membrane, followed by spectrofluorimetric deter-
mination of both drugs, on the solid
surface and the filtered aqueous solution, respectively. AMI shows
low native fluorescence, but its
separation-preconcentration by immobilization (solid-phase
extraction)
on nylon membrane surface provides a
considerable enhancement in fluorescence intensity. The fluores-
cence determination is carried out at
ex = 237, em = 415 nm for FUR; and ex = 365, em = 406 nm for AMI.
The calibration graphs are linear in
the range 3.20 × 10−4 to 0.8 g mL−1 and 1.33 × 10−3 to 4.0
g mL−1 ,
for AMI and FUR, respectively, with a
detection limit of 9.62 × 10−5 and 4.01 × 10−4 g mL−1 (S/N
= 3). The
commonly found excipients in commercial
pharmaceutical formulations do not interfere. The developed
method is successfully applied to the
determination of both drugs in pharmaceutical formulations.