New solid surface fluorescence methodology for lead traces determi- nation using rhodamine B as fluorophore and coacervation scheme: Application to lead quantification in e-cigarette refill liquids

TALÍO, MARÍA CAROLINA; ZAMBRANO, KAREN; KAPLAN, MARCOS; ACOSTA, MARIANO; GIL, RAÚL; LUCONI, MARTA; FERNANDEZ, LILIANA

TALANTA

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 143 p. 315 - 319

0039-9140

P { margin-bottom: 0.21cm; }A new environmental friendlymethodology based on fluorescent signal enhancement of rhodamine Bdye is proposed for Pb(II) traces quantification using apreconcentration step based on the coacervation phenomenon. Acationic surfactant (cetyltrimethylammonium bromide, CTAB) andpotassium iodine were chosen for this aim. The coacervate phase wascollected on a filter paper disk and the solid surface fluorescencesignal was determined in a spectrofluorometer. Experimental variablesthat influence on preconcentration step and fluorimetric sensitivityhave been optimized using uni-variation assays. The calibrationgraph using zero th order regression was linear from 7.4x10-4to 3.4 μg L-1 with a correlation coefficient of 0.999.Under the optimal conditions, a limit of detection of 2.2x10-4μg L-1 and a limit of quantification of 7.4x10-4μg L-1 were obtained. The method showed good sensitivity,adequate selectivity with good tolerance to foreign ions, and wasapplied to the determination of trace amounts of Pb(II) in refillsolutions for e-cigarettes with satisfactory results validated byICP-MS. The proposed method represents an innovative application ofcoacervation processes and of paper filters to solid surfacefluorescence methodology.