ON-LINE METHOD FOR MONTELUKAST DETERMINATION IN BILE SALT MEDIUM WITH FLUORESCENT DETECTION MULTIVARIATE OPTIMIZATION

PERALTA, CECILIA M.; ACOSTA, GIMENA; HENESTROSA, CECILIA; GIL, RAÚL A.; FERNÁNDEZ, LILIANA P.

JOURNAL OF ANALYTICAL CHEMISTRY

MAIK NAUKA/INTERPERIODICA/SPRINGER

Lugar: Kiev; Año: 2022

1061-9348

The present development involved a flow injection strategy using a mini-column of multiwalledcarbon nanotubes and fluorescent detection (λex = 283 nm; λem = 397 nm) for Montelukast(MKT) determination. Optimization technique through experimental design was implemented in order to consider simultaneously different aspects of analysis. Different factors that influence the evaluated analytical responses (sensitivity and sampling frequency) were studied. Taurodeoxycholate bile salt was evaluated as enhancing agent on MKT fluorescence signal. The optimum conditions for MKT determination were: Sample Taurodeoxycholate concentration = 5.0 10−5 mol L−1, Eluent Flow Rate = 0.6 mL min−1 and Sample Flow Rate = 2.2 mL min−1. The method was confirmed to be linear in the range of 0.03?15.00 µg mL−1, with a limit of detection of 11 ng mL−1 and limit of quantification of 30 ng mL−1. The method was successfully applied to MKT determination in pharmaceutical preparations with suitable recoveries between 92.5 and 107.5%