Automated flow system for sildenafil enrichment using surfactant coated solid-phase with fluorescence detection

WANG, CHIEN CHUN; SOMBRA. LORENA; FERNANDEZ, LILIANA

TALANTA

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2012 vol. 98 p. 247 - 252

0039-9140

In this work, Amberlite XAD-1180 resin is used for on-line surfactant-mediated preconcentration of sildenafil as a previous step for its fluorescent detection. In order to activate the column for sildenafil pre-concentration, the cationoc surfactant (hexadecyltrimethylammoniunm bromide, HTAB) is adsorbed onto the resin. In these conditions, sildenafil is retained by HTAB-resin and thenit is eluted with ethanol and analyzed by spectrofluorimetry. Drug-surfactant association produces a considerable fluorescence enhancement, increasing considerably the sensitivity of detection. Therefore, sildenafil can be preconcentrated and quantitatively determined, with a detection limit of 0.2 ng mL-1. The proposed method was successfully applied to the analysis of bulk drug, human urine, tablets, and local herbal medicine. Validation processes were performed by recovering studies and statistical analysis with satisfactory results.