EVALUATION OF CARBON NANOTUBES AS CHIRAL SELECTORS FOR CONTINUOUS-FLOW ENANTIOMERIC SEPARATION OF CARVEDILOL WITH FLUORESCENT DETECTION. DOI 10.1016/j.jpba.2012.06.031

SILVA, RAUL; TALÍO, MARÍA CAROLINA; LUCONI, MARTA; FERNANDEZ, LILIANA

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2012 vol. 70 p. 631 - 635

0731-7085

<!-- @page { margin: 2cm } P { margin-bottom: 0.21cm } --> Single-walled carbon nanotubes (SWNT) are proposed as chiral selectors for separation of carvedilol stereoisomers beginning since its racemic mixture. The novel developed FIA-methodology employs a microcolumn (mC) packed with a few milligrams of SWNT which showed to be effective in S(-) and R(+) carvedilol separation. Attending to spectral properties of analytes, molecular fluorescence was employed in the detection step. Separation of carvedilol enantiomers was achieved in less than 70 s with an acceptable resolution factor of 3.16. Variables that influence the chiral separation such as pH and composition of eluent solution, sample injection volume and flow rate, activation mode of NTs and mass of the same in column has been examined in detail. At optimal operational conditions, well repeatability were achieved using the same column during more of 100 injections, putting in evidence the stability of nanomaterial and the efficacy and versatility of the proposed FIA-configuration. The new methodology was successfully applied to S(-) and R(+) carvedilol quantification in pharmaceutical preparations, resulting an attractive alternative to traditional separative methods being fast, simple, using low cost instrumentation and producing scarce waste.