Immunological changes in IL-10 deficient mice during the course of immunosuppression induced by bacterial endotoxins

CORDOBA MORENO M; FONTANALS A; MARTIRE-GRECO D; FERNÁNDEZ G.C; ISTURIZ M.A; RAMOS M.V; REARTE B.

Buenos Aires

Congreso; LXIII Reunión Anual de la Sociedad Argentina de Inmunología; II Meeting Franco Argentino de Inmunología; IV Reunión de la Sociedad Latinoamericana de Inmunodeficiencias; 2015

SAI

In Sepsis caused by Gram negative, the membrane lipopolysaccharides (LPS) of these bacteria -also called endotoxins- induce a severe immunosuppression, the principal cause of death. We previously demonstrated the glucocorticoids participation in this immunosuppression. Objective: The aim of this study was to evaluate the IL-10 contribution to the immunosuppression and its relationship with glucocorticoids. Methods: Wild type (WT) and IL-10 deficient (KO) BALB/c mice were immunosuppressed with LPS (IS). This was evaluated by cytometry, ELISA and hemagglutination assay. Results: KO mice were more sensitivity to LPS [mortality (LPS 10ug): WT=0/8; KO=8/8]. Also an elevated and sustained level of TNF-α up to 6 hours was observed [mean ± SEM TNF-α (pg/ml): WT=731.8±9; KO=3566±9; p