INDUCED PLURIPOTENT STEM CELL-DERIVED MESENCHYMAL STEM CELL DECREASE ENDOTHELIAL DAMAGE CAUSED BY SHIGA TOXIN TYPE 2

MARTIRE GRECO D; LUZZANI C; CASTILLO L; LA GRECCA A; FURMENTO VA; BIRNBERG WEISS F; LANDONI V.I; MIRIUKA S; FERNÁNDEZ G.C.

Congreso; LXVI Reunion Anual de la Sociedad Argentina de Inmunologia; 2018

SAI

Shiga toxin 2 (Stx2) is key in microangiopathic events that occur in Hemolytic Uremic Syndrome (HUS). Exposure to lipopolysaccharide (LPS) and Stx2, cause endothelial damage in the renal glomerulus inducing one of the most relevant issues that come to kidney failure in this disease. Mesenchymal stem cells (MSC) are multipotent cells that possess known tissue regenerative properties. Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cell (iPSC-MSC) has similar characteristics to Mesenchymal Stem Cell (MSC). Our objective was to study if iPSC-MSC could reduce the endothelial damage/dysfunction caused by LPS and Stx2 exposure. For this purpose, we used an in vitro model of endothelial damage using HUVEC cells incubated with LPS and/or Stx for 24 h. We found that iPSC-MSC exposed to LPS decreased their migratory capacity measured as the migrated area after a wound in the cell monolayer compared to Control cells, but the combination of LPS+Stx reversed this effect (area cm2(x102), Control: 5,1±0,4; LPS: 3,6±0,2*; Stx: 3,8±0,3*; LPS+Stx: 6,5±0,4*,*vs. Control, p