ENDOTHELIAL DIFFERENTIATION FROM INDUCED PLURIPOTENT STEM CELLS AND ITS TIME EXPRESSION OF ENDOTHELIAL MARKERS

MARTIRE GRECO, DAIANA; AMIN GUADALUPE; COLLI CAROLINA; BIRNBERG WEISS, FEDERICO; PITTALUGA, JOSÉ R.; LANDONI V.I; FERNÁNDEZ G.C.; MIRIUKA S

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

Differentiation of induced pluripotent (iPSCs) cells into highly specialized cell types, such as endothelial cells (iECs), neurons, cardiomyocytes, fibroblasts, lung and intestinal cells are technologies related to stem cells that has been growing for many years. Blood vessels are distributed within all tissues of the body and perform various functions. Therefore, the derivation of mature vascular endothelial cells, which restore the lumens of blood vessels, from human pluripotent stem cells is crucial for a multitude of tissue engineering and regeneration applications The aim of this work was to established a protocol to derived induced pluripotent stem cells (FN2.1) to iECS cells and observed the temporary expression of endothelial markers during its differentiation. 250.000 iPSCs cells were cultured with 6uM CHIR at day 0 and B27 (-) and on day 3 we added 10 ng/ ml VEGF for 8 days. We observed that on day 5 endothelial markers began to increased their expression per cell respect to day 0, measured by flow cytometry (percentage (%) CD144: 6 ±0,5; CD34: 4±0,2; CD31: 2±0,1, p