THE LACK OF NET INDUCTION BY KLEBSIELLA PNEUMONIAE IS NOT REVERSED WITH PLATELETS

FEDERICO BIRNBERG- WEISS; JOSE RAMÓN PITTALUGA; JOSELYN CASTRO; LUIS CASTILLO MONTAÑEZ; MARTIRE GRECO, DAIANA; SONIA GOMEZ; VERONICA INÉS LANDONI; GABRIELA CRISTINA FERNÁNDEZ

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Congreso; Reunion conjunta anual 2022; 2022

Klebsiella pneumoniae carbapenemase (Kp)-producing bacteria are associated with significant mortality in immunocompromised patients. We have previously reported that neutrophils (PMN) failed to release neutrophil-extracellular-traps (NET) when challenged with Kp belonging to ST258, whereas Escherichia coli (Eco) was a high NET inducer. It has been reported that PLT activation and PMN:PLT mixed-aggregate formation potrentiate NET induced by LPS and other soluble stimuli. Our aim was to study if the lack of Kp-mediated NET induction can be reversed by the presence of PLT.To assess the effect of both bacteria on PLT activation we measured P-selectin expression by flow cytometry. We found an increment in the percentage of P-selectin-positive PLT when challenged with Kp or Eco (p