INVESTIGADORES
FERNANDEZ Claudio Oscar
artículos
Título:
Site-Specific Copper-Catalyzed Oxidation of α-Synuclein: Tightening the Link between Metal Binding and Protein Oxidative Damage in Parkinson's Disease
Autor/es:
CLAUDIO O. FERNANDEZ
Revista:
INORGANIC CHEMISTRY
Editorial:
AMER CHEMICAL SOC
Referencias:
Lugar: Washington; Año: 2014 p. 4350 - 4358
ISSN:
0020-1669
Resumen:
Amyloidaggregationofα-synuclein(AS)hasbeen linked to the pathological effects associated with IIParkinson?sdisease(PD).CubindsspecificallyattheN-IterminusofASandtriggersitsaggregation.Site-specificCu-catalyzedoxidationofAShasbeenproposedasaplausiblemechanismformetal-enhancedASamyloidformation.InthisIstudy,CubindingtoASwasprobedbyNMRspectroscopy,incombinationwithsyntheticpeptidemodels,site-directedmutagenesis,andC-terminal-truncatedproteinvariants.OurresultsdemonstratethatbothMetresiduesinthemotif15MDVFMconstitutekeystructuraldeterminantsforthehigh-IaffinitybindingofCutotheN-terminalregionofAS.
