Inhibitory effect of short-term bile duct ligation on hepatic cytochrome P450 of bile acid-depleted rats

AGÜERO, RUT M.; FAVRE, CRISTIAN; RODRIGUEZ-GARAY, EMILIO A.

PATHOBIOLOGY

KARGER

Año: 2001 vol. 69 p. 30 - 35

1015-2008

In this study we analyzed the effect resulting from a short-term (1 h) bile duct obstruction in bile acid-depleted or taurocholate-replenished rats on liver cytochrome P450 enzyme system activity. Rats were depleted of endogenous bile acids and then subjected to a biliary obstruction for 1 h. Some of these depleted-obstructed rats were replenished previously to the obstruction with exogenous taurocholic acid (TC) and the others were treated with the solvent alone. To study the isolated effect of the bile acid, other rats were also previously depleted and then replenished with TC but they were obstructed briefly (20 min). CYP3A2-linked activity was evaluated in vivo with the aminopyrine breath test and in vitro by the measurement of nifedipine oxidase microsomal activity. The results suggested that bile flow suppression per se might inhibit this CYP-linked activity and that bile acid retention is not involved at least as a sole determinant. Copyright © 2001 S. Karger AG, Basel.