INVESTIGADORES
DIONISI Hebe Monica
artículos
Título:
Alkali metal ions protect mitochondrial rhodanese against thermal inactivation
Autor/es:
HEBE M. DIONISI; CAROLINA V. ALVAREZ; A. M. VIALE
Revista:
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Editorial:
Elsevier
Referencias:
Lugar: Gran Bretaña; Año: 1999 vol. 361 p. 202 - 206
ISSN:
0003-9861
Resumen:
Incubation of bovine liver mitochondrial rhodanese in dilute, reducing solutions at temperatures ranging between 30 and 45°C conduced to a rapid loss of enzymatic activity. This inactivation was substantially reduced in the presence of millimolar concentrations of alkali metal ions, divalent cations (including Mg21, Ca21, and Ba21) were ineffective. The extent of protection afforded by monovalent cations was highly dependent on their ionic radii, with K1 and Na1 ions being the most effective protective agents. The protection afforded by a number of anions, including thiosulfate, could be totally ascribed to the presence of the accompanying monovalent cation. The overall results indicate that K1 and Na1, at concentrations and temperatures within the physiological range, substantially contribute to the stabilization of the functional structure of rhodanese. © 1999 Academic Press
