Development of an ELA-DRA gene typing method based on pyrosequencing technology

DIAZ S; ECHEVERRÍA MG; IT V; POSIK DM; ROGBERG-MUÑOZ A; PENA NL; PERAL GARCIA P; VEGA-PLA JL; GIOVAMBATTISTA G

TISSUE ANTIGENS

Blackwell Publishing

Año: 2008 vol. 72 p. 464 - 468

0001-2815

Molecular typing of the Equine Lymphocyte Antigen (ELA) class II DRA gene by Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) and Reference Strand-Mediated Conformation Analysis (RSCA), allow identifying eleven ELA-DRA exon 2 sequences, three of which are widely distributed among domestic horse breeds. Herein, describe the development of a pyrosequencing-based method applicable to ELA-DRA typing, by screening samples from eight different horse breeds previously typed by PCR-SSCP. This sequence-based method would be useful in high throughput genotyping of MHC genes in horses and other animal species, making this system interesting as a rapid screening in animal genotyping of immune related genes.