IAL   21557
INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Functional and Biochemical characterization of a Dithiol Glutaredoxin in Trypanosoma cruzi
Autor/es:
MARQUEZ VANINA E; ARIAS DIEGO G.; IGLESIAS ALBERTO A.; GUERRERO SERGIO A.
Lugar:
Casa Pueblo, Punta Ballena, Maldonado
Reunión:
Simposio; Thiol metabolism and redox regulation of cellular functions; 2011
Institución organizadora:
UDELAR-IP Montevideo
Resumen:
Functional
and Biochemical characterization of a Dithiol Glutaredoxin in Trypanosoma
cruzi
Márquez,
Vanina1;
Arias, Diego1; Iglesias, Alberto2; Guerrero,
Sergio1.
1Laboratorio de
Bioquímica Microbiana, 2 Laboratorio de Enzimología Molecular.
Facultad de Bioquímica y Ciencias
Biológicas. Universidad Nacional del Litoral.
Santa Fe, Argentina.
Trypanosoma cruzi,the
causative agent of Chagas disease, is exposed to
several reactive and highly toxic oxygen species (ROS), as consequence of host
defense mechanisms and the incomplete reduction of O2 during aerobic
metabolism. The metabolic pathways that T.
cruzi use to cope with such environmental challenges and redox homeostasis
are matter of our work. Glutaredoxins are oxidoreductases belonging to the
family of thioredoxins that play an important role in the control of cellular
function in several organisms. In this work, we summarized results about
biochemical characterization and experiments performed in order to elucidate
the possible roles played by a dithiol glutaredoxin (TcGrx) in T. cruzi. The
biochemical characterization was performed in enzymatic systems standard for
glutaredoxins. TcGrx was able to
catalyze the reduction of oxidized glutathione and glutathionylaze compounds
using trypanothione as electron donor. Western blotting assays strongly support
the occurrence of a TcGrx in T. cruzi epimastigote. Cultures of T. cruzi over expressing TcGrx were subjected to oxidative stress
by adding glucose oxidase in a glucose containing media. We evaluated cell
viability and verified that TcGrx helps T. cruzi against the action of the oxidants agents generated. Considering
these results, it is possible to suggest protective functions of TcGrx, a new molecular component of the redox metabolism in
trypanosomatids, which is involved in glutathionylation or deglutathionylation of
proteins and recycling of reduced glutathione instead of direct detoxification
of reactive oxygen species.