Boron Neutron Capture Therapy in HT29 colon adenocarcinoma cell line bystander effect and immune genes expression

RODRIGUEZ C; FERREIRA Y; OGLIO R; PERONA M; THORP S; POZZI E; CUROTTO P; DAGROSA MA; THOMASZ L

Novosibirsk

Congreso; 11th Meeting Young member Boron neutrón capture therapy,; 2022

DGBNCT

Colorectal cancer (CRC) is the third commonly diagnosed cancer in the world (Ferlay 2015). In Argentina, CRC is the second most frequent cancer and also the second in mortality. Treatments for this type of cancer include surgery (local excision or colectomy) for curative or palliative purposes, chemotherapy, immunotherapy and radiotherapy. In regards to radiotherapy, it is usually administered to patients who are in advanced stages of the disease, in combination with the treatments described above. However, the effectiveness of photon irradiation is compromised by the physical characteristics of the photon beam itself, resulting in the need to balance local control of tumor growth with the incidence of side effects in surrounding healthy tissue due to the lateral scattering of the beam (Fitzek M, 1999). Boron Neutron Capture Therapy (BNCT), a particle radiotherapy could offer an alternative to photon beam dose limitation since it concentrates the therapeutic dose in the tumor through the selective concentration of boron compounds in the tumor and neutron irradiation reducing the side effects on healthy organs during the treatment. Different nuclear medicine centers have used the amino acid boronphenylalanine (10BPA) in clinical trials for high grade gliomas and melanomas (Busse 2003, Aihara 2006) with almost no toxic effects. BNCT would be particularly important when treating large or multiple liver metastases, as the risk of radiation induced liver metastases might be higher with photon based techniques (Pozzi 2012, 2013). However, it is not much known about how BNCT contributes to these evident therapeutic outcomes from the cellular level and the tumor microenvironment.The aim of this work is to study the bystander effect of BNCT in non-irradiated cells on their capacity to proliferate and migrate and start studying the direct effect on the expression of genes related to immune system response in irradiated HT29 colon carcinoma cells.