Biological Effects of ionizing radiation on tumor microenvironment of thyroid cáncer cell.

RODRIGUEZ C; PERONA M; OGLIO R; GAMBETTA ADRIANA; JUVENAL G; DAGROSA MA; THOMASZ L

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

Sociedad argentina de investigación clínica

Thyroid Cancer (TC) is the most prevalent malignant endocrine system disease. Patients with differentiated thyroid carcinomas have a good prognosis, while some may progress to more aggressive phenotypes such as undifferentiated or anaplastic thyroid carcinoma, which has a very poor prognosis. Ionizing radiation is frequently applied to tumors in patients suffering cancer, with curative or palliative purposes. Nevertheless, tumor resistance and metastasis remain as a clinical concern after radiotherapy. The tumor microenvironment plays a key role in cancer development and progression and ionizing radiation also affects non-tumoral cells in the surrounding tissue. The irradiated cells are believed to communicate signals by affecting the function of non-irradiated cells. We propose to study the effect of soluble factors derived from irradiated thyroid cancer cells on cell proliferation and migration on undifferentiated non-irradiated thyroid cancer cells. Methods: Undifferentiated thyroid carcinoma cells were irradiated with 0, 2, 5 and 8 Gy. After 1, 24, 72 and 96 hours, the supernatants of the irradiated cells were collected and used as conditioned medium for cell proliferation (MTT) and migration (wound) assays. Results: Conditioned medium from irradiated cells after 1 and 24 h have no effect on cell proliferation and migration of non-irradiated cell. After 72 h conditioned medium stimulated significantly cell proliferation of non-irradiated cells by 1.3 fold at 2 Gy, 1.4 fold at 5 Gy and 1.4 fold at 8Gy (p